Speckled microtubules improve tracking in motor-protein gliding assays
نویسندگان
چکیده
منابع مشابه
Displacement-weighted velocity analysis of gliding assays reveals that Chlamydomonas axonemal dynein preferentially moves conspecific microtubules.
In vitro gliding assays, in which microtubules are observed to glide over surfaces coated with motor proteins, are important tools for studying the biophysics of motility. Gliding assays with axonemal dyneins have the unusual feature that the microtubules exhibit large variations in gliding speed despite measures taken to eliminate unsteadiness. Because axonemal dynein gliding assays are usuall...
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In gliding assays, filaments are pulled by molecular motors that are immobilized on a solid surface. By varying the motor density on the surface, one can control the number N of motors that pull simultaneously on a single filament. Here, such gliding assays are studied theoretically using brownian (or Langevin) dynamics simulations and taking the local force balance between motors and filaments...
متن کاملCharacteristics of the motor responsible for the gliding of native microtubules from squid axoplasm.
Nucleotide-dependent movement of native microtubules (nMTs) in squid axoplasm has biochemical and biophysical characteristics of kinesin-driven motility. However, the high vanadate and N-ethylmaleimide sensitivity and the velocity demonstrate that the properties of the native motile system differ considerably from those of purified kinesin preparations.
متن کاملAn N-terminal truncation of the ncd motor protein supports diffusional movement of microtubules in motility assays.
The nonclaret disjunctional (ncd) protein is a kinesin-related microtubule motor protein that is encoded at the claret locus in Drosophila and is required for proper chromosome distribution in meiosis and early mitosis. The protein contains a region with 41% amino acid sequence identity to kinesin heavy chain, but translocates on microtubules with the opposite polarity to kinesin, toward microt...
متن کاملModified Couple Stress Theory for Vibration of Embedded Bioliquid-Filled Microtubules under Walking a Motor Protein Including Surface Effects
Microtubules (MTs) are fibrous and tube-like cell substructures exist in cytoplasm of cells which play a vital role in many cellular processes. Surface effects on the vibration of bioliquid MTs surrounded by cytoplasm is investigated in this study. The emphasis is placed on the effect of the motor protein motion on the MTs. The MT is modeled as an orthotropic beam and the surrounded cytoplasm i...
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ژورنال
عنوان ژورنال: Physical Biology
سال: 2007
ISSN: 1478-3975
DOI: 10.1088/1478-3975/4/1/002